Detect Mycoplasma in cell culture with the e-Myco qPCR Valid kit. This kit allows you detect 70 species with the sensitivity of 10 CFU/ml. But the best part is that you can acheive the results within 90 Minutes. Real-time PCR is an ideal technology for fast and reliable determination of sequence specific DNA. The eMyco VALiD qPCR Kit is an easy way to check your stocks of cell culture for mycoplasma contamination. This probe-based approach, has a FAM-color primer set specific for the highly conserved Mycoplasma 16S-rRNA coding region including M.pneumoniae, M.argnini, M.hyorhinis, M.fermentans, M.orale and A.laidlawii. It also has an internal control HEX-color primer set to ensure that qPCR can occur efficiently.
Mycoplasma is a genus of bacteria which lack a cell wall. Without a cell wall, they are unaffected by penicillin or other antibiotics that target cell wall synthesis. Physically very small (< 1 micron), Mycoplasma are difficult to detect with a conventional microscope or neutralize with common antibiotics. As a result these robust bugs are often found in research laboratories as contaminants in cell culture. Reports indicate that 1/3 or more of all cell cultures are contaminated. Unfortunately, such contamination may induce cellular changes, including chromosome aberrations, changes in metabolism and cell growth. Moreover, severe Mycoplasma infections may destroy a cell line. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotech products.
FOR THE BASIC COST EFFECTIVE KIT
Try the e-Myco KIT. Detect Mycoplasma in cell culture. All the components arrive in a vacuum dried Premix tube. This helps to avoid hydration and oxidation. All you need to do is add your sample. This kit allows you detect 49 species with the sensitivity of 20 CFU/ml.
FOR THE MOST DEMANDING NEEDS
Try e-Myco plus Mycoplasma PCR Detection Kit adds a measure of security when determining cell culture contamination issues. The breadth of detection is dramatically widened to include over 209 species amongst 8 genus, so that nary a mycoplasma can escape detection. In addition a sample control is included to test the veracity of the purified DNA, and is easily detected as a third unique band on an agarose gel (shown below)