M-SolutionTM 1-2 Antibiotics (100x) are a combination of antibiotics, which have been shown to be effective in the elimination of mycoplasma species that account for 90% of the contamination found in cell cultures. M-Solution™ 1-2 Antibiotics (100x) are antibiotic formulations designed to treat Mycoplasma infected cell cultures. M-Solution™ 1 Antibiotic is a solution based on the antibiotic Tiamutin. M-Solution™ 2 Antibiotic is a solution based on the antibiotic Minocycline, which is a member of the Tetracycline group. These two antibiotics have been shown to be effective in eliminating the mycoplasma species frequently present in contaminated cell cultures. Furthermore, the mycoplasmas do not develop resistance to these antibiotics a common occurrence with other antibiotic treatment methods. The two antibiotic solutions are generally used sequentially, in combination. The two antibiotic solutions are used sequentially over a period of 1 week, and the cycle is repeated 2-3 times as required. The treatment is quite easy to perform and when used according to the following protocol, no cytotoxic effects will occur.
ELIMINATION OF MYCOPLASMA IN CELL CULTURE
EFFECTIVE CONTROL OF GRAM (+/) BACTERIA
Mycoplasma is a genus of bacteria which lack a cell wall. Without a cell wall, they are unaffected by penicillin or other antibiotics that target cell wall synthesis. Physically very small (< 1 micron), Mycoplasma are difficult to detect with a conventional microscope or neutralize with common antibiotics. As a result these robust bugs are often found in research laboratories as contaminants in cell culture. Reports indicate that 1/3 or more of all cell cultures are contaminated. Unfortunately, such contamination may induce cellular changes, including chromosome aberrations, changes in metabolism and cell growth. Moreover, severe Mycoplasma infections may destroy a cell line. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotech products.