Detect Mycoplasma in cell culture. All the components arrive in a vacuum dried Premix tube. This helps to avoid hydration and oxidation. All you need to do is add your sample. This kit allows you detect 49 species with the sensitivity of 20 CFU/ml.
Mycoplasma is a genus of bacteria which lack a cell wall. Without a cell wall, they are unaffected by penicillin or other antibiotics that target cell wall synthesis. Physically very small (< 1 micron), Mycoplasma are difficult to detect with a conventional microscope or neutralize with common antibiotics. As a result these robust bugs are often found in research laboratories as contaminants in cell culture. Reports indicate that 1/3 or more of all cell cultures are contaminated. Unfortunately, such contamination may induce cellular changes, including chromosome aberrations, changes in metabolism and cell growth. Moreover, severe Mycoplasma infections may destroy a cell line. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotech products.
The e-Myco™ products contain a set of primers designed to detect the presence of mycoplasma that might contaminate biological materials such as cultured cells. Conventional techniques that are used to detect mycoplasma involve culturing samples on selective media, which needs at least 1 week to obtain results, whereas by performing PCR with this primer set, which is based on conserved 16S rRNA, detection results are obtained in a few hours. The adopted 8-methoxypsoralen (8-MOP) is used to extinguish the template activity of contaminated DNAs. 8-MOP is known to intercalate into double-stranded nucleic acids and form a covalent interstrand crosslink after photo-activation by incident light at wavelength 320-400 nm. An internal control of this product was constructed to identify false negative results in each reaction. The internal control was designed in such a way that the sample primer pair was used to amplify the internal control and target DNA, which were differentiated by size
FOR ENHANCED SENSITIVITY
Try the NEW e-Myco VALiD Kit! E-Myco VALiD PCR Detection Kits have been shown to be a highly sensitive, specific and rapid method for the detection of Mycoplasmas contamination in cell cultures. These kits can identify the five most common cell culture-infecting species of Mycoplasma all the way down to 10 CFU/ml
FOR THE MOST DEMANDING NEEDS
Try e-Myco plus Mycoplasma PCR Detection Kit adds a measure of security when determining cell culture contamination issues. The breadth of detection is dramatically widened to include over 209 species amongst 8 genus, so that nary a mycoplasma can escape detection. In addition a sample control is included to test the veracity of the purified DNA, and is easily detected as a third unique band on an agarose gel (shown below)
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