The FavorPrepâ„¢ Woody Plant Total RNA Purification Mini Kits for  are designed for the purification of total RNA from plant tissues, plant cells and filamentous fungi, using the modified salt precipitation procedure and RNAse inhibitors without.  Plant RNA is quickly and efficiently isolated without the use of hazardous solvents such as phenol. The resulting products are available for downstream applications including RT-PCR, Northern blotting, primer extension and cDNA library construction.

Features
High Purity: OD260/280: >1.9
High Speed: 30-60 minutes.
Safe Use: Eliminates the use of phenol, chloroform,ethidium bromide, and cesium chloride
Scalable protocol: Solution based procedure can be scaled to suit the application. 

Procedure
The sample is broken down by grinding in liquid nitrogen and passed through the filter column to remove cell debris. The resultant lysate is applied to the spin column. The RNA in the lysate binds to glass fiber matrix in the spin column. The optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer.  Finally, the purified total RNA is eluted by RNase-free water.

Expected Yield
Mini: Up to 5-30μg total RNA from young leaves

Applications
RT-PCR
Quantitative Real-Time PCR
Nothern blotting hybridizations
Primer extension
cDNA Synthesis
mRNA Selection
in vitro Translation
Array Analysis

Operation
Centrifuge/Vacuum

Sample Source
Plant tissues and cells, and filamentous fugi

Sample Size
Mini: UP to 100mg fresh plant tissue

Binding Capacity
Mini: Up to 100μg total RNA

Storage Conditions
Stable for 1 year at room temperature at 15-25°C