The FavorPrepâ„˘ Plant Total RNA Purification Mini Kits are equivalant to the Qiagen 74903 and Qiagen 74904 kits. The Kits are designed for the purification of total RNA from plant tissues, plant cells and filamentous fungi, using the modified salt precipitation procedure and RNAse inhibitors without. Plant RNA is quickly and efficiently isolated without the use of hazardous solvents such as phenol. The resulting products are available for downstream applications including RT-PCR, Northern blotting, primer extension and cDNA library construction.
High Purity: OD260/280: >1.9
High Speed: 30-60 minutes.
Safe Use: Eliminates the use of phenol, chloroform,ethidium bromide, and cesium chloride
Scalable protocol: Solution based procedure can be scaled to suit the application.
The sample is broken down by grinding in liquid nitrogen and passed through the filter column to remove cell debris. The resultant lysate is applied to the spin column. The RNA in the lysate binds to glass fiber matrix in the spin column. The optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer. Finally, the purified total RNA is eluted by RNase-free water.
Mini: Up to 5-30μg total RNA from young leaves
Maxi: Up to 50-300μg total RNA from young leaves
Quantitative Real-Time PCR
Nothern blotting hybridizations
in vitro Translation
Plant tissues and cells, and filamentous fugi
Mini: UP to 100mg fresh plant tissue
Mini: Up to 100μg total RNA
Stable for 1 year at room temperature at 15-25Â°C