FavorPrepâ„˘ Tissue Total RNA Midi Purification Kit is equivalant to the Qiagen 75142 and Qiagen 75144 kits. The Kits are designed for purification of total RNA from animal, tissue, animal cultured cells, and bacterial cells using the chaotropic salt-lysis method without the use of hazardous solvents such as phenol. The purified RNA is suitable for direct use in RT-PCR, Northern blotting, primer extension and cDNA library construction.
High Purity: OD260/280: >1.9
Safety: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride.
High Speed: 30-60 minutes
The method uses detergents and a chaotropic salt to lyse cells and inactivate RNase. The RNA in the chaotropic salt is bound to the glass fibre matrix of column. After washing off the contaminants, the purified RNA is eluted by RNase-free water. The entire procedure can be completed about 40~60 minutes.
Quantitative real-time PCR
Nothern blotting hybridizations.
in vitro Translation
Animal Tissues, Animal Cultured Cells, Gram +/- Bacteria
Up to 25mg animal tissues
Up to 1 x10'7 animal cultured cells
Up to1x10'9 bacterial cultured cells
Up to 5 x10'7 Yeast
100~300mg animal tissue
Up to 1 x10'8 animal cultured cell
Up to 1x10'10 bacterial cultured cells
Up to 5 x10'8 Cells
0.5-1g animal tissue
Up to 5 x10'8 animal cultured cells
Up to 5x10'10 bacterial cultured cells
Up to 5 x10'9 Yeast
Mini: Up to 100μg total RNA
Midi: Up to 1mg RNA
Maxi: Up to 2.5mg total RNA
Mini: About 40-60 minutes;Midi: About 60 minutes;Maxi: About 60 minutes
Stable for at room temperature at 15-25Â°C.