The FavorPrepâ„˘ Stool DNA Isolation Kit is equivalant to the Qiagen 51504 Kit. It is designed for the isolation of high quality total DNA from 50~200mg of fresh or frozen stool sample. The inhibitors of the downstream application such as polysaccharides, humic acid, phenolic compounds will be removed by utilizing the DNA binding column and the sequent buffer system in this kit. The entire procedure is performed without the need of phenol-chloroform extraction and is completed within 60 mins. The purified DNA is ready for PCR and other downstream applications.
Time: Rapid isolation of DNA within 60 minutes without phenol/chloroform extraction.
Purity: Eliminate humic acid, polysaccharides, phenol compounds, and enzyme inhibitors
Sample Size: 50~200 mg of fresh or frozen stool sample.
Format: Spin Column
In this simple and rapid process, the stool samples are homogenized and lysed by buffer containing glass beads, Proteinase K and detergents. Special buffer is used to remove debris, proteins, and
polysaccharides by precipitation and other contaminants are washed away by alcohol containing wash buffer. Finally, the purified DNA is eluted by low-salt elution buffer or water.
PCR, Real-Time PCR
Stable for 1 year at room temperature