FavorPrep™ 96-Well GEL/PCR Clean Up DNA Kit is equivalent to the Qiagen 28181 & Qiagen 28183 Kits

The kit provides a high-throughput, rapid and economical method to purify fragmented DNA.  DNA Binding wash and elution steps could be carried out on a vacuum manifold or by centrifugation.  The entire procedure can be completed in 30 minutes and the eluted DNA is ready to use in restriction digestions, ligation, PCR and sequencing reactions

How it works:

The method utilizes a chaotrophic salt to dissolve agarose gel and denature enzymes.  The DNA is absorbed to the fibre matrix in each well of the plate.  Following washing off contaminants, the purified DNA is eluted by low salt buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reactions mixtures without toxic phenol extraction and alcohol precipitation.  Typical recoveries equal 70-85% for gel extraction and 90-95% for PCR cleanup.

Applications
PCR
Fluorescent or Radioactive Sequencing
Restriction Digestion
DNA Labeling
Ligation and Transformation

Sample Size
Up to 300mg agarose gel slice
Up to 100 μl PCR product or other enzymatic reaction

DNA Size Range
70bp~12Kb

Format
96 Wells

Elution Volume
30-50μl

Operation
Centrifugal / vacuum manifold

Binding Capacity
10μg per well 

Handling Time
20 minutes for PCR clean up
30 minutes for Gel Extraction

Expectant Yield (Recovery Rate)
70%-85% for GEL Extraction
90-95% for PCR clean up

Storage Conditions
Stable for 1 year at room temperature 15-25°C. The kit is shipped at ambient temperature.