The FavorPrepâ„˘ Gel/PCR Purification Kit is a cost effective tool that gives you a quick purification where yields are not important. IT is a dual purpose kit designed to recover or concentrate DNA Fragments (70bp-12Kb) from agarose gel or clean PCR or other enzymatic reaction.
If Yields are important we reccomend you use the Gel Purification (FAGPK001) or PCR Clean-Up (FAPCK001) kits
The method uses a chaotropic salt, guanidine thiocyanante to dissolve the agarose gel and denature enzymes. The DNA fragment in the chaotropic salt is bound to the glass fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by a low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from reaction mixture without phenol extraction and alcohol precipitation.
Verasatile: Purify DNA from Agarose Gels, PCR or other enzymatic reactions
Recovery: 60-75% fo Gel extraction; 80-85% for PCR clean-up
Fast: 25 mins for Gel extraction 15 mins for PCR clean-up
Sample Size: uptp 30mg for Gel slices; upto 100μl PCR product or other enzymatic reaction
Size: 70bp-12kb DNA
Binding capacity: Upto 10ug
Safey: No need for ethanol precipitation or phenol/chloroform extractions
Shelf Life: Stable for 1 year at room temperature 15-25Â°C
Shipped at ambient temperature