The FavorPrepâ„˘ Plasmid DNA Extraction Midi Kits is equivalant to the Qiagen 12143 and 12145 kits. Order the Endotoxin Free (EF) kit using the following code :FAPDE002_EF
Format: Gravity flow
Purity: Equal to that obtained by 2x CsCl gradient centrifugation
Expectant Yield: upto 500μg of transfection grade plasmid
Time Required: Under 120 minutes
The kits include pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from 50-100 ml of bacterial culture. When using the standard protocol, the entire process can be completed in 120 minutes or less and up to 500μg of plasmid can be expected. The purity of purified plasmid DNA is equal to that obtained by 2x CsCl-gradient centrifugation and suitable for all molecular biological applications
Plasmid DNA Extraction Midi/Maxi Kits can be stored at room temperature (15-25C). After adding RNase A, PM 1 Buffer should be stored at 4C and is stable for six months. Other buffers and columns can be stored dry for up to 1 year at room temperature (15-25C).