EntryPepâ„˘ kit provides a fast and simple method for delivering a protein of choice into a wide variety of cells via conjugation with a cell permeable polypeptide sequence(CP). Itâ€™s a convenient method for covalent modification of 0.2â€“2 mg of protein, ie an antibody â€“ by a specific short polypeptide sequence. Click here for more details
This cross-linking is based on the formation of a stable thioether bond. The resulting complex becomes cell membrane permeable but retains the biological activity of the modified protein (e.g. antigen recognition by antibody). This complex can be used in various assays such as visualization or functional modulation of intracellular proteins in living cells. The protocol is appropriate for all proteins containing thiol groups.
■ The efficiency of internalization is higher than with common DNA transfection methods.
■ The procedure is much faster than conventional transfection-based expression analysis.
■ The conjugate is equally distributed among all treated cells.
■ Functional effects can be measured immediately after internalization of the conjugate.
■ Protein levels within the cell are not dependant on the efficiency of transcription/translation.
EntryPepâ„˘ Kit delivers
■ Protein complexes
■ Chemically modified proteins
■ All other compounds with -SH group
EntryPepâ„˘ Kit Applications
■ Analysis of sub-cellular distribution of dye-labeled proteins in living cells
■ Visualization of intracellular proteins with CP-conjugated antibodies or other specific ligands
■ Detection of co-localization of multiple CP-conjugated proteins in living cells
■ Verification of protein-protein interactions in a cellular environment (results from two-hybrid assays)
■ Inhibition or activation of intracellular processes with a specific protein
■ Functional analysis of proteins that are difficult to express in mammalian cells (toxicity/low expression levels)
■ Rapid assessment of activity of recombinant proteins in a cellular environment
■ Evaluation of intracellular effects of chemically modified proteins (which cannot be done using DNA transfection based techniques)