Lipopolysaccharides (LPS) is a major constituent of the outer membrane of gram-negative bacteria. Some of the LPS is a disease-causing endotoxin; it is also important in classification of serum type. Commonly used method is the complicated hot phenol-water extraction that takes long period of time. LPS Extraction Kit is designed for rapid and convenient extraction of LPS. It even enables LPS extraction from small volume of bacterial cells. Download the Protocol here

LPS extraction from bacterial cells
Distribution of bacteria by patterns of carbohydrate chain length
Immunostimulatory effect of the extracted LPS from bacteria
Very simple and convenient steps
Rapid reaction time (within 60 minutes)

Format: Solution type (Lysis buffer and Purification buffer)
Sample Source: Bacterial Cells
Sample Size: The yield of LPS extraction is proportional to volume of culture. The yield of LPS is at its maximum when 3-5ml of culture is used. The optimal culture volume is 1-2ml at OD600 of 0.8-1.2
Prep-Time: <60 Mins including a lysis step
Rehydration Volume: 50-200ul

STORAGE
4 Degrees Celcius