RedSafe Nucleic Acid Staining Solution (20,000x) is a NEW and SAFE nucleic acid stain, an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. Download the Protocol
RedSafe emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and the other at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafe bound to DNA is centered at 537 nm. RedSafe Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr. The staining protocol for RedSafe Nucleic Acid Staining Solution (20,000x) is similar to that for EtBr. Compared to EtBr. RedSafe Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. So it is wise to choose RedSafe Nucleic acid Stainig Solution (20,000x) instead of EtBr for detecting nucleic acid in agarose gels.
Used for detecting double-strand DNA and single-stranded RNA Alternative to the ethidium bromide stainingAs sensitive as EtBr or more visualization of DNA(>50 ng) under visible lightNon-toxic, non-mutagenic and non-carcinogenic No hazard waste
Stored at room temperature. This product has a stable shelf-life of 12 Months, Longer if stored at 4C
RedSafe Nucleic Acid Staing Solution (20,000x) is non-carcinogenic but may cause skin and eye irritations. Please wear gloves when working with the product. To ensure good sensitivy make sure the gels are not thicker than 0.5cm. RedSafe is regarded as Non-Toxic however we do recommend that you follow the rules and regulations of your local water boards.